Kribbella jiaozuonensis sp. nov., a novel actinomycete isolated from soil

A novel actinobacterium, designated strain NEAU-THZ27 T , was isolated from soil collected from the Cornel peak in Jiaozuo, Henan Province, PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics of the strain coincided with those of members of the genus Kribbella . The results of 16S rRNA gene sequence analysis showed that strain NEAU-THZ27 T belongs to the genus Kribbella and was most closely related to Kribbella podocarpi YPL1 T (98.96 %), Kribbella karoonensis Q41 T (98.89 %), Kribbella aluminosa HKI 0478 T (98.86%) and Kribbella hippodromi S1.4 T (98.85 %), similarities to other type strains of species of the genus Kribbella were found to be less than 98.7 %. Phylogenetic analyses using the 16S rRNA gene sequence and multilocus sequence analysis using the concatenated gene sequences of the gyrB , rpoB , recA , relA and atpD genes all showed that the strain formed a separate branch in the genus Kribbella . The cell wall contained ll-diaminopimelic acid as the major diamino acid and the whole-cell hydrolysates were ribose and glucose. The major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinone was MK-9(H 4 ). Major fatty acids were iso-C 16 : 0 , iso-C 14 : 0 and anteiso-C 15 : 0 , these chemotaxonomic data supported the affiliation of strain NEAU-THZ27 T o the genus Kribbella . The DNA G+C content was 68.0 mol%. Furthermore, the strain could be clearly distinguished by concatenated gene genetic distances, the combination of DNA-DNA hybridization results and some phenotypic characteristics. Therefore, it is proposed that strain NEAU-THZ27 T represents a novel species of the genus Kribbella , for which the name Kribbella jiaozuonensis sp. nov. is proposed. The type strain is NEAU-THZ27 T (=CGMCC 4.7504 T =DSM 105535 T ).