Open AccessRhodosalinus halophilus sp. nov., isolated from a marine solar saltern, and emended description of the genus Rhodosalinus
Author(s) -
Zhenwei Liu,
Tao-Jie Wang,
Hao Yang,
Dan Lü,
ZongJun Du
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003646
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , phosphatidylglycerol , botany , strain (injury) , phylogenetics , genomic dna , glycolipid , ribosomal rna , microbiology and biotechnology , bacteria , gene , genetics , biochemistry , phospholipid , anatomy , membrane , phosphatidylcholine
A novel Gram-stain-negative, rod-shaped, beige-coloured, motile, facultatively anaerobic bacterium, designated as E84 T , was isolated from sediment sampled from a marine solar saltern in Wendeng, PR China. Phylogenetic analysis based on 16S rRNA gene sequencing showed that Rhodosalinus sediminis WDN1C137 T was the closest phylogenetic relationship, with 16S rRNA gene sequence similarity of 96.9 %. Optimal growth occurred at 33-37 °C (range, 20-40 °C), at pH 7.5 (pH 7.0-8.5) and with 6.0 % (w/v) NaCl (0.5-20.0 %). The sole respiratory quinone was Q-10. The major fatty acids were summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ), C 18 : 0 and cyclo C 19 : 0 ω8 c . The polar lipids were phosphatidylglycerol, one unidentified glycolipid, two unidentified phospholipids and two unidentified lipids. The genomic DNA G+C content of strain E84 T was 69.8 mol%. Based on the results of physiological, genotypic, phylogenetic and chemotaxonomic analyses, we concluded that strain E84 T represents a novel species of the genus Rhodosalinus , for which the name Rhodosalinus halophilus sp. nov. is proposed. The type strain is E84 T (=KCTC 52697 T =MCCC 1H00231 T ).