Open AccessRamlibacter humi sp. nov., isolated from tropical forest soil
Author(s) -
Xianjiao Zhang,
Guang-Da Feng,
Qing Yao,
Yonghong Wang,
Song-Zhen Yang,
Honghui Zhu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003641
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , phosphatidylglycerol , strain (injury) , botany , microbiology and biotechnology , bacteria , gene , phosphatidylcholine , biochemistry , genetics , phospholipid , anatomy , membrane
A Gram-stain-negative, aerobic and non-motile strain, designated 18x22-1 T , was isolated from a forest soil sample collected from Limushan Nature Reserve in Hainan Province, PR China. Growth occurred at 15-37 °C and pH 6.0-8.0 without NaCl. The 16S rRNA gene sequence analyses showed that strain 18x22-1 T was closely related to Ramlibacter tataouinensis DSM 14655 T (98.5 %), followed by Ramlibacter henchirensis DSM 14656 T (97.9 %) and other Ramlibacter species and formed a stable cluster with R. tataouinensis DSM 14655 T , R. henchirensis DSM 14656 T , Ramlibacter solisilvae JCM 19319 T and Ramlibacter rhizophilu s CCTCC AB 2015357 T . Results of chemotaxonomic analyses showed that ubiquinone-8 (Q-8) was the major respiratory quinone, and the major fatty acids (>10 % of the total amounts) were C 16 : 0 and C 17 : 0 cyclo. The major polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminopholipids and four unidentified phospholipids. The draft genome sequence was 4.47 Mb long with a G+C content of 68.9 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between strain 18x22-1 T and four closely related type strains were in the range of 79.3-82.3 % and 21.9-25.1 %, respectively. The results of phenotypic, phylogenetic and chemotaxonomic analyses supported that strain 18x22-1 T represents a novel species of the genus Ramlibacter , for which the name Ramlibacterhumi sp. nov. is proposed. The type strain is 18x22-1 T (=GDMCC 1.1584 T =KCTC 52922 T ).