Steroidobacter soli sp. nov., isolated from farmland soil

A Gram-stain-negative bacterial strain, designated JW-3 T , was isolated from a soil sample collected from farmland in Yantai, Shandong Province, PR China. Cells of strain JW-3 T are motile rods and strictly aerobic, showing catalase- and oxidase-positive reactions. Strain JW-3 T could grow at 16-37 °C (optimum, 30 °C), at pH 6.0-9.0 (pH 7.0) and in the presence of 0-1 % (w/v) NaCl (0.5 %, in Luria-Bertani broth). The major fatty acids were summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ; 35.5 %), iso-C 16 : 0 (16.7 %) and C 12 : 0 (10.8 %). The major respiratory quinone was ubiquinone-8 (Q8). The polar lipids of strain JW-3 T consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, four unidentified phospholipids, two unidentified lipids, two unidentified glycolipids and a partial unidentified aminophospholipid. Strain JW-3 T was most closely related to Steroidobacter agariperforans KA5-B T with 97.67 % 16S rRNA gene sequence similarity. Results of phylogenetic analyses, based on 16S rRNA gene sequencing, showed that strain JW-3 T forms a distinct phylogenic lineage within the genus Steroidobacter of the family Sinobacteraceae . The DNA G+C content of strain JW-3 T was 62.57 mol%, based on its draft genome sequence. Average nucleotide identity values and digital DNA-DNA hybridization values for draft genomes, between strain JW-3 T and strain KA5-B T , were 84.54 and 30.80 %, respectively. Based on its phenotypic, chemotaxonomic and molecular features, and DNA-DNA hybridization results, strain JW-3 T represents a novel species of the genus Steroidobacter , for which the name Steroidobactersoli sp. nov. is proposed. The type strain is JW-3 T (=CCTCC AB 2018184 T =KCTC 62820 T ).