Open AccessStreptomyces otsuchiensis sp. nov., a biosurfactant-producing actinobacterium isolated from marine sediment
Author(s) -
Takeshi Terahara,
Takuya Naemura,
Yukiko Nampo,
Takeshi Kobayashi,
Chiaki Imada,
Moriyuki Hamada,
Tomohiko Tamura
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003638
Subject(s) - biology , 16s ribosomal rna , streptomyces , peptidoglycan , strain (injury) , diamino acid , microbiology and biotechnology , actinobacteria , actinomycetales , streptomycetaceae , botany , bacteria , cell wall , biochemistry , gene , genetics , gene sequence , anatomy
A novel actinobacterium producing biosurfactant, designated OTB305 T , was isolated from marine sediment sampled at Otsuchi Bay, Iwate Prefecture, Japan and its taxonomic position was examined using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences exhibited that strain OTB305 T was closely related to Streptomyces bohaiensis JCM 19630 T (98.8 %) and Streptomyces lonarensis DSM 42084 T (98.8 %). The chemotaxonomic characteristics of strain OTB305 T corresponded to those of the genus Streptomyces as follows: the diamino acid of the cell-wall peptidoglycan was ll-diaminopimelic acid; whole-cell hydrolysates contained glucose and lacked characteristic major sugars; the predominant isoprenoid quinones were MK-9(H 8 ) and MK-9(H 6 ); the polar lipids were phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unidentified phospholipid; the major cellular fatty acids were iso-C 16 : 0 , C 16 : 0 and C 16 : 1 ω 7 c ; and the genomic DNA G+C content was 72.83 mol%. However, genomic relatedness analysis based on the average nucleotide identity and some phenotypic characteristics revealed that strain OTB305 T was distinguished from closely related Streptomyces species. Therefore, strain OTB305 T represents a novel species of the genus Streptomyces , for which the name Streptomyces otsuchiensis sp. nov. is proposed. The type strain is OTB305 T (=NBRC 113255 T =TBRC 9682 T ).