Open AccessDeinococcus arcticus sp. nov., isolated from Silene acaulis rhizosphere soil of the Arctic tundra
Author(s) -
Xinpeng Wang,
Changming Liu,
Yong Yu,
Huirong Li,
ZongJun Du,
DaShuai Mu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003636
Subject(s) - biology , rhizosphere , 16s ribosomal rna , deinococcus , phylogenetic tree , tundra , botany , strain (injury) , ribosomal rna , genus , microbiology and biotechnology , bacteria , deinococcus radiodurans , arctic , gene , ecology , genetics , anatomy
A Gram-stain-negative, non-motile, aerobic, short rod-shaped bacterium, designated OD32 T , was isolated from a soil sample taken from the rhizosphere of Silene acaulis collected from the Arctic tundra. The novel strain, OD32 T , was able to grow at 15-37 °C (optimum, 33 °C), pH 6.5-9.5 (pH 7.5-8.0) and 0-0.5 % NaCl (0 %, w/v). A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain OD32 T was most closely related to the genus Deinococcus ; it shared the highest sequence similarity (95.7 %) with Deinococcus grandis ATCC 43672 T . Genomic DNA G+C content of the strain OD32 T was 68.2 mol%. The predominant respiratory quinone was menaquinone 8 (MK-8). The major fatty acids were summed feature 3 (C 16 : 1 ω 7 c /C 16 : 1 ω 6 c ), C 15 : 1 ω 6 c , C 17 : 1 ω 6 c , C 17 : 0 , C 16 : 0 and iso-C 17 : 0 . Based on phylogenetic inference and genotypic characteristics, strain OD32 T represents a novel species of the genus Deinococcus , for which the name Deinococcus arcticus sp. nov. is proposed. The type strain is OD32 T (=KCTC 33972 T =CICC 24671 T ).