Open AccessTabrizicola alkalilacus sp. nov., isolated from alkaline Lake Dajiaco on the Tibetan Plateau
Author(s) -
Dorji Phurbu,
Hui Wang,
Qian Tang,
Huibin Lü,
Han Zhu,
SiPing Jiang,
Peng Xing,
Qinglong L. Wu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003635
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , genome size , phosphatidylglycerol , bacteria , genomic dna , microbiology and biotechnology , botany , dna , gene , genome , genetics , phospholipid , anatomy , membrane , phosphatidylcholine
A novel Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium was isolated from Lake Dajiaco on the Tibetan Plateau. Strain DJC T grew without NaCl and tolerated up to 3 % (w/v) NaCl. Growth occurred at pH 6.0-10.0 (optimum, pH 7.0-8.0) and 15-37 °C (optimum, 25-30 °C). Vitamins were not required for growth. The main polar lipids of strain DJC T were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was Q-10. The major fatty acid was C 18 : 1 ω7 c . Genome sequencing revealed a genome size of 4.61 Mbp and a G+C content of 62.9 mol%. Analysis of 16S rRNA sequences showed that strain DJC T belonged to the genus Tabrizicola , with the closest neighbour Tabrizicola aquatica RCRI19 T (97.5 %). DNA-DNA relatedness between strain DJC T and the closest phylogenetically related strain T. aquatica RCRI19 T was 40.8 %. Stain DJC T was clearly distinguished from the type strain mentioned above through phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristic comparisons. Based on its phenotypic and chemotaxonomic characteristics, strain DJC T could be classified as a representative of a novel species of the genus for which the name Tabrizicola alkalilacus sp. nov. is proposed. The type strain is DJC T (=CICC 24242 T =KCTC 62173 T ).