Open AccessMicromonospora acroterricola sp. nov., a novel actinobacterium isolated from a high altitude Atacama Desert soil
Author(s) -
Lorena Carro,
Patrycja Golińska,
Imen Nouioui,
Alan T. Bull,
José Mariano Igual,
Bárbara A. Andrews,
Hans Peter Klenk,
Michael Goodfellow
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003634
Subject(s) - biology , micromonospora , 16s ribosomal rna , strain (injury) , phylogenetic tree , diaminopimelic acid , botany , actinomycetales , microbiology and biotechnology , phylogenetics , bacteria , gene , genetics , streptomyces , anatomy
A Micromonospora strain, designated 5R2A7 T , isolated from a high altitude Atacama Desert soil was examined by using a polyphasic approach. Strain 5R2A7 T was found to have morphological, chemotaxonomic and cultural characteristics typical of members of the genus Micromonospora. The cell wall contains meso - and hydroxy -diaminopimelic acid, the major whole-cell sugars are glucose, ribose and xylose, the predominant menaquinones MK-10(H 4 ), MK-10(H 6 ), MK-10(H 8 ) and MK-9(H 6 ), the major polar lipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown glycolipid, and the predominant cellular fatty acids iso -C 16 : 0 , iso -C 15 : 0 and 10-methyl C 17 : 0 . The digital genomic DNA G+C content is 72.3 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain 5R2A7 T was closely related to Micromonospora coriariae DSM 44875 T (99.8 %) and Micromonospora cremea CR30 T (99.7 %), and was separated readily from the latter, its closest phylogenetic neighbour, based on gyrB and multilocus sequence data, by low average nucleotide identity (92.59 %) and in silico DNA-DNA relatedness (51.7 %) values calculated from draft genome assemblies and by a range of chemotaxonomic and phenotypic properties. Consequently, strain 5R2A7 T is considered to represent a novel species of Micromonospora for which the name Micromonospora acroterricola sp. nov. is proposed. The type strain is 5R2A7 T (=LMG 30755 T =CECT 9656 T ).