Open AccessErythrobacter suaedae sp. nov., isolated from a rhizosphere mudflat of a halophyte (Suaeda japonica)
Author(s) -
Soon Dong Lee,
YoungJu Kim,
In Seop Kim
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003625
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , botany , strain (injury) , rhizosphere , microbiology and biotechnology , bacteria , gene , genetics , anatomy
The taxonomic status of a Gram-reaction-negative, aerobic, motile, rod-shaped bacterium, designated strain GH3-15 T , was examined by a polyphasic approach. The strain, which was isolated from the rhizosphere mudflat of a halophyte at the seashore of Gangwha Island, Republic of Korea, was found to belong to the family Erythrobacteraceae based on 16S rRNA gene sequences. The closest phylogenetic neighbour was Erythrobacter xanthus SM1501 T (98.3 % sequence similarity). Levels of 16S rRNA gene sequence similarity of strain GH3-15 T to other members of the family Erythrobacteracae were <97.1 %. The respiratory quinone was Q-10. The major fatty acids were C 17 : 1 ω6 c , C 18 : 1 ω7 c , C 15 : 0 2-OH, 11-methyl C 18 : 1 ω7 c and C 17 : 0 . The polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The novel isolate exhibited growth at 20-40 °C, at pH 5-9, and in the presence of 1-7 % (w/v) NaCl. DNA relatedness between strain GH3-15 T and its closet relative was 32.9±8.8 %. On the basis of phenotypic, chemotaxonomic and DNA-DNA hybridization data, in addition to a distinct phylogenetic position, strain GH3-15 T (=KCTC 62380 T =JCM 32445 T ) represents a novel species of the genus Erythrobacter, for which the name Erythrobactersuaedae sp. nov. is proposed.