Open AccessPseudomonas huaxiensis sp. nov., isolated from hospital sewage
Author(s) -
Jiangyue Qin,
Yu Feng,
Xiaoyun Lu,
Zhiyong Zong
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003622
Subject(s) - biology , pseudomonas , 16s ribosomal rna , microbiology and biotechnology , phylogenetic tree , strain (injury) , pseudomonas putida , genetics , gene , bacteria , anatomy
A novel Gram-negative, obligate aerobic, rod-shaped, motile by one or two polar flagella, non-spore-forming bacterial strain, WCHPs060044 T , was isolated from the wastewater treatment plant at West China Hospital in Chengdu, PR China. Analysis of its 16S rRNA gene sequence showed that strain WCHPs060044 T belonged to the genus Pseudomonas with the highest similarity to Pseudomonas alkylphenolica KL28 T (99.73 %), Pseudomonas donghuensis HYS T (99.52 %) and Pseudomonas wadenswilerensis CCOS 846 T (99.38 %). Phylogenomic analysis based on 107 core gene sequences demonstrated that WCHPs060044 T was a member of the Pseudomonas putida group but was distant from all closely related species. Whole-genome comparisons, using average nucleotide identity based on blast (ANIb) and in silico DNA-DNA hybridization (isDDH), confirmed low genome relatedness to its close phylogenetic neighbours [below the recommended thresholds of 95 % (ANIb) and 70 % (isDDH) for species delineation]. Phenotypic characterization tests showed that the utilization of l-arginine, l-fucose, d-galacturonic, d-glucuronic acid, inositol and sorbitol in combination could distinguish this strain from other related species of the genus Pseudomonas . Therefore, based on genetic and phenotypic evidence, strain WCHPs060044 T represents a novel species, for which the name Pseudomonas huaxiensis sp. nov. is proposed. The type strain is WCHPs060044 T (GDMCC 1.1396 T =JCM 32907 T =CCTCC AB 2018120 T =CNCTC 7663 T ).