Open AccessPseudomonas daroniae sp. nov. and Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK
Author(s) -
Victoria Bueno-Gonzalez,
Carrie Brady,
Sandra Denman,
Sarah J. Plummer,
Joël Allainguillaume,
Dawn L. Arnold
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003615
Subject(s) - biology , rpob , 16s ribosomal rna , phylogenetic tree , microbiology and biotechnology , pseudomonas , multilocus sequence typing , botany , phylogenetics , bacteria , gene , genotype , genetics
Twenty-two cream-coloured bacterial strains were isolated from oak trees affected by acute oak decline (AOD) in Southern England. Isolates were Gram-negative, motile, slightly curved rods, aerobic, non-spore-forming, catalase positive and oxidase positive. 16S rRNA gene sequence analysis placed the strains in two separate phylogenetic clusters in the Pseudomonas straminea group, with Pseudomonas flavescens as the closest phylogenetic relative. Multilocus sequence analyses of the gyrB , rpoD and rpoB genes supported the delineation of the strains into two separate taxa, which could be differentiated phenotypically and chemotaxonomically from each other, and their closest relatives. Average nucleotide identity and in silico DNA-DNA hybridization values revealed percentages of genome similarity below the species threshold (95 and 70 %, respectively) between the two taxa and the closest relatives, confirming their novel species status. Therefore, on the basis of this polyphasic approach we propose two novel Pseudomonas species, Pseudomonasdaroniae sp. nov. (type strain FRB 228 T =LMG 31087 T =NCPPB 4672 T ) and Pseudomonasdryadis sp. nov. (type strain FRB 230 T =LMG 31087 T =NCPPB 4673 T ).