Open AccessAquibacillus sediminis sp. nov., a moderately halophilic bacterium isolated from saltern soil
Author(s) -
Jae-Chan Lee,
Kyung-Sook Whang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003599
Subject(s) - biology , halophile , 16s ribosomal rna , diamino acid , microbiology and biotechnology , strain (injury) , peptidoglycan , phylogenetic tree , bacteria , diaminopimelic acid , phylogenetics , cell wall , botany , gene , gene sequence , biochemistry , genetics , anatomy
A Gram-stain-positive, moderately halophilic bacterium, designated strain BH258 T , was isolated from solar saltern sediment sampled at Shinan in the Republic of Korea. Cells of strain BH258 T were found to be strictly aerobic, motile, endospore-forming rods which could grow at 15-45 °C (optimum, 35 °C), at pH 5.5-9.0 (pH 7.0) and at salinities of 0.5-20 % (w/v) NaCl (7-10%). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BH258 T belongs to the genus Aquibacillus , showing highest sequence similarity to Aquibacillus koreensis BH30097 T (96.1 %), Aquibacillus albus YIM 93624 T (95.9 %), Aquibacillus halophilus B6B T (95.6 %) and Aquibacillus salifodinae WSY08-1 T (95.1 %). The predominant isoprenoid quinone was identified as menaquinone-7, and the cell-wall peptidoglycan was found to contain meso -diaminopimelic acid as the diagnostic diamino acid. The major fatty acids were identified as anteiso-C 15 : 0 , iso-C 16 : 0 , anteiso-C 17 : 0 and iso-C 15 : 0 . The major polar lipids were identified as phosphatidylglycerol, diphosphatidylglycerol and three unidentified phospholipids. The DNA G+C content of this novel isolate was determined to be 37.35 mol%. On the basis of the results of phylogenetic, phenotypic and chemotaxonomic analyses in this study, strain BH258 T is considered to represent a novel species of the genus Aquibacillus , for which the name Aquibacillus sediminis sp. nov. is proposed. The type strain is BH258 T (=KACC 18680 T =NBRC 111875 T ).