Open AccessArthrobacter celericrescens sp. nov., isolated from forest soil
Author(s) -
Rui Yan,
Dongli Liu,
Yansong Fu,
Ye Zhang,
Hanxun Ju,
Junwei Zhao,
Xiangjing Wang,
Ji Zhang,
Wensheng Xiang
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003597
Subject(s) - 16s ribosomal rna , biology , arthrobacter , rpob , strain (injury) , phylogenetic tree , microbiology and biotechnology , coccus (insect) , genomic dna , botany , bacteria , gene , genetics , anatomy
A novel bacterial strain, designated NEAU-SA2 T , was isolated from forest soil collected from the Zhangjiajie city, Hunan Province, PR China and characterised using a polyphasic approach. The cells were aerobic, Gram-stain-positive, non-flagellated and rod-coccus-shaped. The strain grew optimally at 28 °C, pH 7.0 and with 0 % NaCl (w/v). Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Arthrobacter and was closely related to Arthrobacter cupressi DSM 24664 T (98.89 %) and Arthrobacter silvisoli CCTCC AB 2017271 T (98.41 %), which was further confirmed by multilocus sequence analysis. The major cellular fatty acids were anteiso -C 15 : 0 , anteiso -C 17 : 0 and C 16 : 0 ; MK-9(H 2 ) was the predominant respiratory quinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The peptidoglycan type was A3α, and the cell-wall sugars were glucose and galactose. The genomic G+C content of strain NEAU-SA2 T was 67.04 mol%. The average nucleotide identity values between NEAU-SA2 T and A. cupressi DSM 24664 T and A. silvisoli CCTCC AB 2017271 T were 88.57-90.94 %. The digital DNA-DNA hybridisation values between strain NEAU-SA2 T and its most closely related species were 37.00 and 41.10 %, respectively, again indicating that they belong to different taxa. Therefore, strain NEAU-SA2 T represents a novel species of the genus Arthrobacter , for which the name Arthrobacter celericrescens sp. nov. is proposed. The type strain is NEAU-SA2 T (=DSM 106718 T =CCTCC AB 2017272 T ).