Open AccessRoseovarius amoyensis sp. nov. and Muricauda amoyensis sp. nov., isolated from the Xiamen coast
Author(s) -
Guizhen Li,
Qiliang Lai,
Peisheng Yan,
Zongze Shao
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003595
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phosphatidylethanolamine , phosphatidylglycerol , microbiology and biotechnology , phylogenetic tree , botany , gene , phospholipid , phosphatidylcholine , biochemistry , anatomy , membrane
Strain GCL-8 T and GCL-11 T were isolated from Guanyinshan, a sandy beach on the Xiamen coast. Cells of the two strains were Gram-stain-negative, catalase-positive, oxidase-positive, rod-shaped and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains GCL-8 T and GCL-11 T belonged to the genera Roseovarius and Muricauda , with the highest sequence similarities to Roseovarius indicus B108 T (96.5%) and Muricauda antarctica CGMCC 1.12174 T (97.3%), respectively. The major fatty acids of strain GCL-8 T were summed feature 8 (C 18:1 ω7 c /ω6 c ), C 19:0 cyclo ω8 c , C 16:0 and C 17:0 , while the major fatty acids of strain GCL-11 T were iso-C 15:1 G, iso-C 15:0 and iso-C 17:0 3-OH. The G+C contents of the chromosomal DNA of strains GCL-8 T and GCL-11 T were 64.1 mol% and 45.67 mol%, respectively. The respiratory quinones of strains GCL-8 T and GCL-11 T were Q-10 and MK-6, respectively. The polar lipids of strain GCL-8 T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, one unidentified aminolipid, three unidentified phospholipids and three unidentified polar lipids. The polar lipids of strain GCL-11 T were phosphatidylethanolamine, seven unidentified phospholipids and four unidentified polar lipids. The combined genotypic and phenotypic data show that strains GCL-8 T and GCL-11 T represent two novel species within genera Roseovarius and Muricauda , for which the names Roseovariu samoyensis sp. nov. and Muricauda amoyensis sp. nov. are proposed, with type strains GCL-8 T (=MCCC 1A11651 T = KCTC 52430 T ) and GCL-11 T (=MCCC 1A11649 T =KCTC 52432 T ).