Open AccessFerruginivarius sediminum gen. nov., sp. nov., isolated from a marine solar saltern
Author(s) -
Shuo Wang,
Zi-Hui Ye,
Nannan Wang,
Da-Shuai Mu,
ZongJun Du
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003589
Subject(s) - biology , 16s ribosomal rna , strain (injury) , botany , phosphatidylglycerol , genus , microbiology and biotechnology , bacteria , genetics , phospholipid , phosphatidylcholine , membrane , anatomy
A novel Gram-stain-negative, facultatively anaerobic, oxidase-negative, catalase-positive bacillus, designated WD2A32 T , was isolated from a marine solar saltern in the coast of Weihai, Shandong Province, PR China. Strain WD2A32 T grew optimally at 37-40 °C (range, 20-45 °C) and pH 7.0-7.5 (range, 6.5-8.0) and was tolerant to 6-8 % (w/v) NaCl (range, 2-12 %). The 16S rDNA of strain WD2A32 T was most similar (93.5 %) to that of Rhodovibrio salinarum DSM 9154 T (the type species of the genus Rhodovibrio ), followed by Limimonas halophila DSM 25584 T (92.4 %; the type species of the genus Limimonas ). The similarities to the type strains of the genera Pelagibius and Limibacillus were less than 91.0 %. The draft genome sequence of strain WD2A32 T contained 72 contigs (>507 bp) of 4 237 996 bp with a DNA G+C content of 65.5 mol%. The major polar lipids of strain WD2A32 T were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and unidentified aminolipids; major cellular fatty acids were summed feature 8 (C 18 : 1 ω7 c /C 18 : 1 ω6 c ) and C 19 : 0 cyclo ω8 c ; the major respiratory quinone was Q-10. On the basis of the results from chemotaxonomic, physiological and biochemical analyses, strain WD2A32 T represents a novel species of a novel genus in the family Rhodospirillaceae , for which the name Ferruginivarius sediminum gen. nov., sp. nov. has been proposed. The type strain is WD2A32 T (=KCTC 52888 T =MCCC 1H00201 T ).