Open AccessPaenimaribius caenipelagi gen. nov., sp. nov., isolated from a tidal flat
Author(s) -
Sooyeon Park,
Sung Min Won,
JungHoon Yoon
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003588
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , alphaproteobacteria , genus , diamino acid , dendrogram , botany , phylogenetics , bacteria , gene , genetics , gene sequence , genetic diversity , anatomy , population , demography , sociology
A Gram-stain-negative, strictly aerobic, non-motile and ovoid- or rod-shaped bacterial strain, JBTF-M29 T , was isolated from tidal flat sediment sampled from the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain JBTF-M29 T clustered with the type strains of Maribius species and this cluster joined the clade comprising Pseudomaribius and Palleronia species. Strain JBTF-M29 T exhibited 16S rRNA gene sequence similarity values of 96.2-96.6 % to the type strains of three Maribius species, of 96.4 % to the type strain of Pseudomaribius aestuariivivens and of 93.9-94.7 % to the type strains of three Palleronia species. In the UPGMA dendrogram based on the ANI values of genomic sequences, strain JBTF-M29 T formed an evolutionary lineage independent of the genera Maribius and Palleronia and some other taxa. Strain JBTF-M29 T contained Q-10 as the predominant ubiquinone and C 18 : 1 ω 7 c and C 18 : 0 as the major fatty acids. The major polar lipids of strain JBTF-M29 T were phosphatidylglycerol and one unidentified aminolipid. The DNA G+C content of strain JBTF-M29 T was 64.5 mol%. The differences in fatty acid and polar lipid profiles and other differential phenotypic properties including nitrate reduction and casein hydrolysis made it reasonable to distinguish strain JBTF-M29 T from the genera Maribius , Pseudomaribius and Palleronia . Therefore, on the basis of the polyphasic taxonomic data presented here, strain JBTF-M29 T constitutes a new genus and species within the class Alphaproteobacteria , for which the name Paenimaribius caenipelagi gen. nov., sp. nov. is proposed. The type strain is JBTF-M29 T (=KACC 19867 T =NBRC 113548 T ).