Open AccessFudania jinshanensis gen. nov., sp. nov., isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) in China
Author(s) -
Wentao Zhu,
Jing Yang,
Shan Lu,
XinHe Lai,
Dong Jin,
Ji Pu,
Xiaoxia Wang,
YuYuan Huang,
Sihui Zhang,
Ying Huang,
Yuanmeihui Tao,
Zhihong Ren,
Xiaomin Wu,
Xiaoyan Zhang,
Jianqing Xu,
Jianguo Xu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003586
Subject(s) - biology , 16s ribosomal rna , diamino acid , phylogenetic tree , peptidoglycan , phylogenetics , ribosomal rna , strain (injury) , microbiology and biotechnology , rpob , rhamnose , bacteria , gene , genetics , gene sequence , biochemistry , mannose , anatomy
Two hitherto unknown bacteria (strains 313 T and 352) were recovered from the faeces of Tibetan antelopes on the Tibet-Qinghai Plateau, PR China. Cells were rod-shaped and Gram-stain-positive. The optimal growth conditions were at 37 °C and pH 7. The isolates were closely related to Actinotignum sanguinis (92.6 % 16S rRNA gene sequence similarity), Arcanobacterium haemolyticum (92.5 %), Actinotignum schaalii (92.4 %), Actinobaculum massiliense (92.2 %) and Flaviflexus huanghaiensis (91.6 %). Phylogenetic analyses showed that strains 313 T and 352 clustered independently in the vicinity of the genera Actinotignum , Actinobaculum and Flaviflexus , but could not be classified clearly as a member of any of these genera. Phylogenomic analysis also indicated that strains 313 T and 352 formed an independent branch in the family Actinomycetaceae . The major cellular fatty acids of the strains were C 16 : 0 and C 18 : 1 ω9 c . The polar lipids comprised diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylglycerol, phosphatidylinositol and five unidentified components. The peptidoglycan contained lysine, alanine and glutamic acid. The respiratory quinone was absent. The whole-cell sugars included glucose and rhamnose. The DNA G+C content of strain 313 T was 60.6 mol%. Based on the low 16S rRNA gene sequence similarities, its taxonomic position in the phylogenetic and phylogenomic trees and its unique lipid pattern, we propose that strains 313 T and 352 represent members of a novel species in a new genus, for which the name Fudania jinshanensis gen. nov., sp. nov. is proposed. The type strain is 313 T (=CGMCC 4.7453 T =DSM 106216 T ).