Open AccessReclassification of 'Polyangium brachysporum' DSM 7029 as Schlegelella brevitalea sp. nov.
Author(s) -
Biao Tang,
Yucong Yu,
Junheng Liang,
Youming Zhang,
Xiaoying Bian,
XiaoYang Zhi,
Xiaoming Ding
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003571
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , ribosomal rna , genomic dna , fatty acid , gene , biochemistry , botany , genetics , anatomy
Strain DSM 7029, isolated from a soil sample in Greece, can produce antitumour glidobactins, and has been found, as a heterologous host, to produce useful nonribosomal peptide synthetase-polyketide synthase hybrid molecules known as epothilones. This strain was originally named ' Polyangium brachysporum ' of the family Polyangiaceae and the order Myxococcales . However, phylogenetic analysis of the 16S rRNA gene sequence of strain DSM 7029 indicated that it was clustered with members of Schlegelella . Significant growth occurred at 25-42 °C, pH 5.0-10.0 and in the presence of 0-0.2 % (w/v) NaCl. The predominant ubiquinone was Q-8. The major fatty acids were C 16 : 1 ω7 c /C 16 : 1 ω6 c , C 16 : 0 and C 18 : 1 ω7 c . The G+C content of genomic DNA was 67.51 mol%. The strain was clearly distinguishable from other neighbouring Schlegelella members and genera Caldimonas and Zhizhongheella , using phylogenetic analysis, fatty acid composition data and a range of physiological and biochemical characteristics and genome analysis. Therefore, strain DSM 7029 represents a novel species of the genus Schlegelella , for which the name Schlegelella brevitalea sp. nov. is proposed.