Open AccessSphingosinithalassobacter portus gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from surface seawater
Author(s) -
Buce Hetharua,
Derong Min,
Hu Liao,
Wei Guo,
Xiaolan Lin,
Hong Xu,
Yun Tian
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003566
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , genus , phosphatidylglycerol , botany , glycolipid , microbiology and biotechnology , bacteria , gene , phosphatidylcholine , biochemistry , phospholipid , genetics , anatomy , membrane
A Gram-stain-negative strain, designated FM6 T , was isolated from surface seawater sampled at the port in Xiamen, PR China. Strain FM6 T showed less than 96.3 % 16S rRNA gene sequence similarity to the type strains of species with validly published names. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FM6 T belonged to the family Sphingomonadaceae and was closely related to species of the genera Sphingomonas (96.3 %) and Stakelama (96.0 %). Ubiquinone-10 was the predominant respiratory quinone. Cells were motile with a single polar flagellum. Growth occurred at temperatures from 20 to 45 °C (optimum, 30 °C), at pH values between pH 6.0 and 8.0 (optimum, pH 7.0) and in 0-4.0 % (w/v) NaCl (optimum, 1.0-1.5 %). Predominant polar lipids were sphingoglycolipid, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, five unidentified glycolipids and five unidentified polar lipids. The major fatty acids were summed feature 8 (containing C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ). The DNA G+C content of the type strain was 63.8 mol%. On the basis of the results of phylogenetic analysis, combined with phenotypic and chemotaxonomic data, strain FM6 T is considered to represent a novel species in a new genus in the family Sphingomonadaceae for which the name Sphingosinithalassobacter portus gen. nov., sp. nov. is proposed. The type strain of Sphingosinithalassobacter portus gen. nov., sp. nov. is FM6 T (=MCCC 1K03501 T =JCM 32714 T ).