Open AccessWeissella cryptocerci sp. nov., isolated from gut of the insect Cryptocercus kyebangensis
Author(s) -
Jun Heo,
Moriyuki Hamada,
Hayoung Cho,
Hang-Yeon Weon,
Jeong-Seon Kim,
Sheng Hong,
Soojin Kim,
Soon-Wo Kwon
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003564
Subject(s) - biology , 16s ribosomal rna , weissella , peptidoglycan , microbiology and biotechnology , phylogenetic tree , strain (injury) , ribosomal rna , bacteria , gene , genetics , anatomy , leuconostoc , lactobacillus
A taxonomic study of a Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, catalase-negative bacterium, isolated from the gut of an insect, Cryptocercus kyebangensis collected from the mountainous area of Seoraksan, Yangyang-gun, Republic of Korea, was conducted. Its 16S rRNA gene sequence showed high similarity values to Weissella ghanensis LMG 24286 T (95.9 %), Weissella beninensis 2L24P13 T (95.9 %), Weissella fabalis M75 T (95.7 %) and Weissella fabaria 257 T (95.7 %). The phylogenetic tree indicated that the novel organism formed a cluster with W. ghanensis LMG 24286 T , W. beninensis 2L24P13 T , W. fabalis M75 T and W. fabaria 257 T . The G+C content was 41.1 mol% on the basis of the whole-genome sequence. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, two unidentified aminophospholipids, one unidentified phospholipid and four unidentified lipids. The major cellular fatty acids were C 18 : 1 ω 9 c , C 16 : 0 , C 14 : 0 , summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) and summed feature 8 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ). The cell-wall peptidoglycan was of A4 α type with the interpeptide bridge of Gly-d-Glu. Based on these results, strain 26KH-42 T could be classified as a novel species of the genus Weissella , for which the name Weissellacryptocerci sp. nov. is proposed. The type strain is 26KH-42 T (=KACC 18423 T =NBRC 113066 T ).