Lactobacillus huachuanensis sp. nov., isolated from Chinese traditional pickle

A Gram-stain-positive bacterial strain, 395-6.2 T , was isolated from traditional pickle in Heilongjiang Province, PR China. The bacterium was characterised by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, fatty acid methyl ester (FAME) analysis, determination of DNA G+C content, average nucleotide identity (ANI) analysis, in silico DNA-DNA hybridisation ( is DDH) and an analysis of phenotypic features. Analysis of the 16S rRNA gene sequence showed that strain 395-6.2 T was phylogenetically related to Lactobacillus farciminis , Lactobacillus formosensis , Lactobacillus futsaii , Lactobacillus crustorum , Lactobacillus nuruki , Lactobacillus heilongjiangensis , Lactobacillus musae , Lactobacillus nantensis , Lactobacillus mindensis , Lactobacillus kimchiensis , Lactobacillus zhachilii , Lactobacillus alimentarius , Lactobacillus bobalius , Lactobacillus kimchii and Lactobacillus paralimentarius . Strain 395-6.2 T exhibited 95.7-99.4 % 16S rRNA gene sequence similarities, 85.0-94.0 %  pheS gene sequence similarities, 94.2-98.0 %  rpoA gene sequence similarities to type strains of phylogenetically related species. ANI and is DDH values between strain 395-6.2 T and type strains of phylogenetically related species were 77.9-87.1 % and 22.5-33.5 %, respectively. Based upon the data obtained in the present study, a novel species, Lactobacillus huachuanensis sp. nov., is proposed and the type strain is 395-6.2 T (=CCM 8927 T =NCIMB 15188 T =LMG 31179 T ).