Open AccessParacoccus tegillarcae sp. nov., isolated from the gastrointestinal tract of a blood cockle (Tegillarca granosa)
Author(s) -
June-Young Lee,
Dong-Wook Hyun,
Ji-Hyun Yun,
Myeong Ho Jung,
Na-Ri Shin,
Jin-Woo Bae
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology (print)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003561
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , strain (injury) , sequence analysis , phosphatidylglycerol , biochemistry , gene , phosphatidylcholine , phospholipid , anatomy , membrane
A novel bacterial isolate, designated as strain BM15 T , was isolated from the gastrointestinal tract of a blood cockle, Tegillarca granosa , which was collected from the foreshore of Beolgyo-eup, Republic of Korea. Strain BM15 T was Gram-stain-negative, non-motile, strictly aerobic and short-rod-shaped. Optimum growth of the isolate occurred at 20 °C, in the presence of 4 % (w/v) NaCl and at pH 6. The 16S rRNA gene sequence analysis showed that strain BM15 T belonged to the genus Paracoccus and had more than 97 % 16S rRNA gene sequence similarity to ' Paracoccus zhejiangensis ' J6 (97.40 % similarity) and Paracoccus lutimaris HDM-25 T (97.04 %). The polar lipid profile of strain BM15 T comprised phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an unidentified glycolipid and two unidentified lipids. The predominant respiratory quinone was ubiquinone-10. The major cellular fatty acid (>20 %) was summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ). The complete genome sequence of strain BM15 T comprised 3,759,866 bp with 62.2 mol% G+C content. The results of the phylogenetic, phenotypic and genotypic analyses indicated that strain BM15 T represents a novel species in the genus Paracoccus , for which the name Paracoccus tegillarcae is proposed. The type strain is BM15 T (=KCTC 72032 T =JCM 33289 T ).