Lujinxingia litoralis gen. nov., sp. nov. and Lujinxingia sediminis sp. nov., two new representatives in the order Bradymonadales

In this study, two bacterial strains designated B210 T and SEH01 T , isolated from coastal sediment sampled in Weihai, PR China, were characterized using a polyphasic approach. Strains were Gram-stain-negative, facultative anaerobic, rod-shaped and motile. According to the results of phylogenetic analyses based on their 16S rRNA genes, these two strains should be classified under the order Bradymonadales and they both show <90 % sequence similarities with Bradymonas sediminis FA350 T . Moreover, strain B210 T showed 98.6 % sequence similarity to strain SEH01 T . Genomic characteristics including average nucleotide identity and in silico DNA-DNA hybridization values clearly separated strain B210 T from strain SEH01 T . The sole quinone of these two strains was menaquinone MK-7, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid. Iso-C 15 : 0 was the major fatty acid in both strains B210 T and SEH01 T , and iso-C 14 : 0 3-OH was also a major fatty acid for strain SEH01 T . Based on the polyphasic analysis, these two strains represent two novel species of a new genus within the family Bradymonadaceae . Consequently, the novel genus Lujinxingia gen. nov. is proposed, containing two new species Lujinxingia litoralis gen. nov. sp. nov. and Lujinxingia sediminis sp. nov., with strain B210 T (=KCTC 42951 T =CGMCC 1.16770 T ) and strain SEH01 T (=KCTC 42950 T =DSM 101859 T ) as the type strains, respectively.