Flavobacterium silvisoli sp. nov., isolated from forest soil

During a study of the Kyonggi University soil bacterial diversity, an aerobic, non-motile, Gram-stain-negative, non-spore-forming, rod-shaped, yellow pigmented bacterium, designated strain RD-2-33 T was isolated. Strain RD-2-33 T grew optimally at 28-35 °C and pH 7.0-7.5; hydrolysed gelatin and DNA; and tolerated 1 % of NaCl. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain RD-2-33 T clustered with the genus Flavobacterium . The closest member was Flavobacterium dankookense ARSA-19 T (97.1 % sequence similarity) and Flavobacterium cheonhonense ARSA-15 T (96.7 %). Sole respiratory quinone was MK-6. The major polar lipids were phosphatidylethanolamine and an unidentified polar lipid. The major cellular fatty acids were iso-C 15 : 0 , iso-C 17 : 0 3-OH, and iso-C 15 : 1 G. The DNA G+C content was 38.6 mol%. The average nucleotide identity (ANI) and in silico DNA-DNA hybridisation relatedness between strain RD-2-33 T and Flavobacterium dankookense DSM 25687 T were 75.2 and 19.3 %, respectively. Based on the polyphasic and phylogenetic data, strain RD-2-33 T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium silvisoli sp. nov. is proposed. The type strain is RD-2-33 T (=KEMB 9005-742 T =KACC 21178 T =NBRC 113789 T ).