Open AccessStreptomyces dangxiongensis sp. nov., isolated from soil of Qinghai-Tibet Plateau
Author(s) -
Binglin Zhang,
ShuKun Tang,
Ruiqi Yang,
Ximing Chen,
Dongming Zhang,
Wei Zhang,
Shi-Weng Li,
Tuo Chen,
Guangxiu Lu,
Paul Dyson
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003550
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , streptomyces , diamino acid , genomic dna , diaminopimelic acid , microbiology and biotechnology , soil microbiology , botany , bacteria , gene , gene sequence , biochemistry , genetics , anatomy
A novel actinobacterial strain, designated Z022 T , isolated from a soil sample collected from Dangxiong in Tibet Autonomous Region (PR China), was determined by polyphasic taxonomic approach. The organism had chemotaxonomic and morphological properties consistent with its classification in the genus Streptomyces. Strain Z022 T showed high similarity value to Streptomyces lucensis NBRC 13056 T (98.87 %) and S. achromogenes subsp . achromogenes NBRC 12735 T (98.68 %) based on the 16S rRNA gene phylogenetic tree. The genomic DNA G+C content of strain Z022 T based on the genome sequence was 72.16 mol%. DNA-DNA relatedness values between strain Z022 T and strain Streptomyces lucensis NBRC 13056 T was 23.7±1.3 % and signicantly lower than 70 %. Chemotaxonomic data revealed that strain Z022 T possessed MK-9(H 8 ) and MK-9(H 6 ) as the predominant menaquinone, ll-diaminopimelic acid as the diagnostic diamino acid, and galactose, glucose, xylose and ribose as whole cell sugars. Diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE) were the predominant polar lipids; anteiso-C 15 : 0 , iso-C 16 : 0 , and anteiso-C 17 : 0 were the major fatty acids. On the basis of these genotypic and phenotypic data, it is proposed that isolate Z022 T (=JCM 31053 T =CGMCC 4.7273 T ) should be classified in the genus Streptomyces as Streptomyces dangxiongensis sp. nov.