Open AccessCellulomonas algicola sp. nov., an actinobacterium isolated from a freshwater alga
Author(s) -
Hideki Yamamura,
T. Hayashi,
Moriyuki Hamada,
Takashi Kohda,
Yukihiko Serisawa,
Kazuyo MatsuyamaSerisawa,
Youji Nakagawa,
Misa Otoguro,
Fujitoshi Yanagida,
Tomohiko Tamura,
Masayuki Hayakawa
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003549
Subject(s) - diamino acid , biology , 16s ribosomal rna , peptidoglycan , strain (injury) , microbiology and biotechnology , thermophile , fatty acid , phylogenetic tree , bacteria , biochemistry , gene , gene sequence , genetics , anatomy
An actinomycete strain, TKZ-21 T , was isolated from a freshwater alga ( Chetophoraceae ) collected from the Takizawa River, Yamanashi, Japan, and examined using a polyphasic taxonomic approach. Cells were Gram-stain positive, aerobic, non-sporulating, motile, and coccoid or short rod-shaped. The strain grew in the presence of 0-4 % (w/v) NaCl, between pH 6-9.4, and over a temperature range of 15-40 °C, with optimum growth at 30 °C. The peptidoglycan type of strain TKZ-21 T was A4β, containing l-ornithine as diagnostic diamino acid and d-glutamic acid as the interpeptide bridge. The predominant menaquinone was MK-9(H 4 ). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, ninhydrin-positive glycolipid, and unidentified phospholipids. The major cellular fatty acids were anteiso-C 15 : 0 and anteiso-C 17 : 0 , and the DNA G+C content was 75.6 mol%. On the basis of 16S rRNA gene sequence analysis, strain TKZ-21 T was closely related to Cellulomonas fimi (98.5 % sequence similarity) and Cellulomonas biazotea (98.3 %). The genome orthoANI value between strain TKZ-21 T and C. biazotea and C. fimi were 84.7 and 84.2 %, respectively. On the basis of fatty acid and MALDI-TOF MS profile analysis, phylogenetic analyses, genomic analysis, and phenotypic data, it is proposed that the isolate be classified as a representative of a novel species of the genus Cellulomonas , with the name Cellulomonas algicola sp. nov. The type strain is TKZ-21 T (=NBRC 112905 T =TBRC 8129 T ).