Open AccessAmycolatopsis suaedae sp. nov., an endophytic actinomycete isolated from Suaeda maritima roots
Author(s) -
Tanatorn Chantavorakit,
Paweena Suksaard,
Atsuko Matsumoto,
Kannika Duangmal
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003546
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , arabinose , botany , strain (injury) , mycelium , diamino acid , microbiology and biotechnology , diaminopimelic acid , gene , biochemistry , gene sequence , fermentation , xylose , anatomy
An actinomycete strain, designated 8-3EHSu T , was isolated from surface-sterilised roots of Suaeda maritima, collected from Petchburi province, Thailand. Taxonomic position of strain 8-3EHSu T was studied using a polyphasic approach. Phylogenetic determination based on the 16S rRNA gene sequence similarity showed that strain 8-3EHSu T belongs to the genus Amycolatopsis, with the highest sequence similarity to Amycolatopsis jiangsuensis KLBMP 1262 T (96.9 %). The colony of strain 8-3EHSu T was yellowish white. Long straight mycelium breaking down into fragments were observed. Growth occurred at temperatures 15-45 °C and at pH 6.0-10.0. The strain contained meso-diaminopimelic acid, arabinose, galactose and mannose in whole-organism hydrolysates. The predominant menaquinone was MK-9(H4). The major fatty acids were C16 : 0, iso-C16 : 0 and iso-C15 : 0. Polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, two unknown amino lipids and an unknown lipid. The G+C content of genomic DNA was 71.8 mol%. A phylogenetic tree based on 16S rRNA sequences showed that the strain 8-3EHSu T formed a distinct evolutionary linage within the genus Amycolatopsis. Based on analysis results of physiological, biochemical and chemotaxonomic data, strain 8-3EHSu T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis suaedae sp. nov. is proposed. The type strain is 8-3EHSu T (=TBRC 8488 T =NBRC 113449 T ).