Open AccessReclassification of Streptomyces rimosus subsp. paromomycinus as Streptomyces paromomycinus sp. nov.
Author(s) -
Hisayuki Komaki,
Tomohiko Tamura
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003545
Subject(s) - biology , microbiology and biotechnology , actinomycetales , streptomyces , streptomycetaceae , subspecies , bacteria , genetics , paleontology
Streptomyces rimosus is currently composed of two subspecies: Streptomyces rimosus subsp. rimosus and Streptomyces rimosus subsp. paromomycinus. The 16S rRNA gene similarity between type strains of these two subspecies is 99.03 %, whereas that between S. rimosus subsp. paromomycinus and Streptomyces chrestomyceticusis 100 %. To assess the taxonomic status of S. rimosus subsp. paromomycinus, genome sequencing was performed on the type strains of S. rimosus subsp. paromomycinus and S. chrestomyceticus. Digital DNA-DNA hybridization values between S. rimosus subsp. paromomycinus NBRC 15454 T and S. rimosus subsp. rimosus ATCC 10970 T and between S. rimosus subsp. paromomycinus NBRC 15454 T and S. chrestomyceticus NBRC 13444 T were 35.4 and 59.9 %, respectively, which are less than the thresholds for bacterial species delineation and indicate that S. rimosus subsp. paromomycinus is not S. rimosus, but an independent species different from S. rimosus and S. chrestomyceticus. In addition, phenotypic data also support that S. rimosus subsp. paromomycinus is distinct from S. chrestomyceticus. Therefore, S. rimosus subsp. paromomycinus should be reclassified as a novel species, for which we propose the name Streptomyces paromomycinus sp. nov. The type strain is NBRC 15454 T (=ATCC 14827 T =DSM 41429 T =JCM 4541 T =JCM 4871 T =NRRL 2455 T =VKM Ac-605 T ).