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Indiicoccus explosivorum gen. nov., sp. nov., isolated from an explosives waste contaminated site
Author(s) -
Yash Pal,
Shanmugam Mayilraj,
Mohit Paul,
Peter Schümann,
Srinivasan Krishnamurthi
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003541
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , peptidoglycan , microbiology and biotechnology , phylogenetics , lineage (genetic) , botany , bacteria , gene , genetics , anatomy
A pink-pigmented, Gram-stain-positive, aerobic, coccoid-shaped bacterial strain, designated as S5-TSA-19 T , was isolated from an explosives contaminated site in Panchkula, Haryana, India. The 16S rRNA gene sequencing blast analysis indicated that the strain is a member of the family Planococcaceae with the highest sequence similarity to Planomicrobium soli XN13 T (96.1 %), followed by Planococcus maitriensis S1 T (95.6 %), Planococcus plakortidis DSM 23997 T (95.6 %), Planomicrobium flavidum ISL-41 T (95.6 %), Planococcus rifietoensis M8 T (95.5 %), Planococcus salinus LCB217 T (95.5 %) and Planococcus maritimus DSM 17275 T (95.5 %). Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences (based on a conserved set of 400 proteins) retrieved the strain in a distinct branch indicating a separate lineage within the family Planococcaceae. Strain S5-TSA-19 T had a distinctive chemotaxonomic pattern comprising A4α type peptidoglycan based on l-Lys-d-Asp, iso-C15 : 0 as the major fatty acid, absence of phosphatidylethanolamine as a major lipid and MK-7 and MK-6 as the major menaquinones, differentiating it from the genera Planococcus and Planomicrobium, thus supporting the findings of molecular phylogeny. Further, strain S5-TSA-19 T was able to biotransform hexahydro-1,3,5,-trinitro-1,2,5-triazine (RDX) into nitrite derivatives under aerobic conditions in 2-4 days, whereas the closest reference strains did not possess this property. On the basis of polyphasic taxonomic characterization and a phylogenomics approach, strain S5-TSA-19 T is proposed as the type strain of a novel species in a novel genus for which the name Indiicoccus explosivorum gen. nov., sp. nov. is proposed (=JCM 31737 T =KCTC 33871 T =MTCC 12608 T ).

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