Open AccessSandaracinomonas limnophila gen. nov., sp. nov., a new member of the family Cytophagaceae isolated from a freshwater mesocosm
Author(s) -
Wen-Ming Chen,
Cheng-Ye Cai,
Shih-Yi Sheu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003532
Subject(s) - biology , spermidine , 16s ribosomal rna , phylogenetic tree , strain (injury) , gene , microbiology and biotechnology , botany , genetics , biochemistry , anatomy , enzyme
A bacterial strain designated FSY-15 T was isolated from a freshwater mesocosm in Taiwan and characterised using a polyphasic taxonomic approach. Cells of strain FSY-15 T were Gram-negative, aerobic, non-spore forming, non-motile rods and formed orange coloured colonies. Growth occurred at 20–30 °C (optimum, 25 °C), at pH 6–7.5 (optimum, pH 7) and with 0–0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain FSY-15 T formed a phylogenetic lineage in the the family Cytophagaceae . Strain FSY-15 T was most closely related to the genera Pseudarcicella and Arcicella , and the levels of 16S rRNA gene sequence identity with respect to members of related genera are less than 94.1 %. Strain FSY-15 T showed less than 68.8 % average nucleotide identity and less than 24.7 % digital DNA–DNA hybridisation identity compared to the type strains of related genera within the family Cytophagaceae . The predominant fatty acids were iso-C 15 : 0 , C 16 : 1 ω 5c and the major hydroxyl fatty acid was iso-C 15 : 0 3-OH. The major isoprenoid quinone was MK-7 and the DNA G+C content was 35.8 mol%. The major polar lipids were phosphatidylethanolamine and several uncharacterised aminophospholipid, aminolipid, phospholipid and lipid. The major polyamine was spermidine. On the basis of the genotypic and phenotypic data, strain FSY-15 T represents a novel species of a new genus in the family Cytophagaceae , for which the name Sandaracinomonas limnophila gen. nov., sp. nov. is proposed. The type strain is FSY-15 T (=BCRC 81011 T =LMG 29732 T =KCTC 52445 T ).