Open AccessChengkuizengella marina sp. nov., isolated from deep-sea sediment of the Pacific Ocean
Author(s) -
Junpeng Yang,
Qiliang Lai,
Xianzhi Liao,
Qin Zhao,
Zongze Shao
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003530
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , oxidase test , microbiology and biotechnology , genus , botany , bacteria , gene , biochemistry , genetics , anatomy , enzyme
A taxonomic study was carried out on strain YPA3-1-1 T , which was isolated from deep-sea sediment of the Pacific Ocean. The bacterium was Gram-stain-positive, oxidase-positive, catalase-negative, rod-shaped and spore-forming. Growth was observed at salinities of 1.0-6.0 % and at temperatures of 10-40 °C. The isolate could degrade gelatin and aesculin. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YPA3-1-1 T belonged to the genus Chengkuizengella, with the highest sequence similarity to the only typespecies, Chengkuizengella sediminis J15A17 T (98.5 %). The estimated average nucleotide identity and DNA-DNA hybridization values between strain YPA3-1-1 T and C. sediminis J15A17 T were 88.1 and 35.0 %, respectively. The cell wall of strain YPA3-1-1 T contained meso-diaminopimelic acid. The principal fatty acids (>10 %) were iso-C16 : 0 (35.5 %) and anteiso-C15 : 0 (17.5 %). The G+C content of the chromosomal DNA was 33.1 mol%. The respiratory quinone was determined to be MK-7 (100 %). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, glycolipid and three unidentified phospholipids. The combined genotypic and phenotypic data show that strain YPA3-1-1 T represents a novel species within the genus Chengkuizengella, for which the name Chengkuizengella marina sp. nov. is proposed, with the type strain YPA3-1-1 T (=MCCC 1A14042 T =KCTC 43019 T ).