Open AccessLongirhabdus pacifica gen. nov., sp. nov., isolated from a deep-sea hydrothermal sediment in the West Pacific Ocean
Author(s) -
Rou-Wen Chen,
Jing Zhang,
Yuan-Qiu He,
Kexin Wang,
Cun Li,
Lijuan Long
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003526
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , deep sea , genus , hydrothermal vent , genomic dna , bacteria , microbiology and biotechnology , botany , gene , hydrothermal circulation , biochemistry , anatomy , genetics , paleontology , fishery
A novel Gram-stain-negative bacterium, designated as SCSIO 06110 T , was isolated from a deep-sea sediment of the West Pacific Ocean. Cells were 0.5-0.8 µm in width and 3.0-4.0 µm in length, spore-forming, rod-shaped with peritrichous flagella. Positive for catalase and urease, negative for oxidase and nitrate reduction. Growth occurred at 15-37 °C, pH 6-9 and 1-5 % (w/v) NaCl, with optimum growth at 28 °C, pH 7 and 3 % (w/v) NaCl. MK-7 was the only menaquinone. The strain possessed diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids. Iso-C 16 : 0 , iso-C 15 : 0 and iso-C 14 : 0 were the major fatty acids. The novel isolate clustered with genera in the family Paenibacillaceae , but formed a separated branch with the closest relative Chengkuizengella sediminis J15A17 T (91.1 % sequence similarity) when compared in a phylogenetic analysis of 16S rRNA gene sequences. The DNA G+C content of strain SCSIO 06110 T was 38.5 mol%. Based on the polyphasic data presented, a new genus, Longirhabdus gen. nov., is proposed in the family Paenibacillaceae with the type species Longirhabdus pacifica sp. nov. and the type strain SCSIO 06110 T (=DSM 105158 T =CGMCC 1.16550 T ).