Microbacterium bovistercoris sp. nov., a novel actinomycete isolated from cow dung

A Gram-stain-positive, aerobic actinomycete strain, designated NEAU-LLE T , was isolated from cow dung collected from Shangzhi, Heilongjiang Province, north-east China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLE T belonged to the genus Microbacterium , with highest sequence similarity to Microbacterium pseudoresistens DSM 22185 T (98.2 %). The predominant menaquinones were MK-13 and MK-14. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid. The major fatty acids were identified as anteiso-C 15 : 0 , iso-C 16 : 0 and iso-C 17 : 0 . However, DNA-DNA relatedness and physiological and biochemical data showed that strain NEAU-LLE T could be distinguished from its closest relative. Therefore, strain NEAU-LLE T represents a novel species of the genus Microbacterium , for which the name Microbacteriumbovistercoris sp. nov is proposed, with NEAU-LLE T (=CCTCC AA 2018025 T =JCM 32662 T ) as the type strain.