Open AccessNakamurella antarctica sp. nov., isolated from Antarctica South Shetland Islands soil
Author(s) -
Xuyang Da,
Yiming Zhao,
Ruichen Zheng,
Liqiu Wang,
Xulu Chang,
Yumin Zhang,
Jian Yang,
Fang Peng
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003507
Subject(s) - biology , 16s ribosomal rna , rhamnose , peptidoglycan , phylogenetic tree , strain (injury) , psychrophile , shetland , diamino acid , botany , phylogenetics , bacteria , microbiology and biotechnology , cell wall , gene , galactose , genetics , biochemistry , gene sequence , oceanography , anatomy , geology
A pale-yellow bacterial strain, designated S14-144 T , was isolated from tundra soil sampled near the Antarctic Peninsula, South Shetland Islands (62° 22' 34″ S, 59° 42' 34″ W). The cells were strictly aerobic, Gram-stain-positive, non-motile and coccoid-shaped. Growth occurred at 4-28 °C, at pH 5.0-9.0 and in the presence of 0-5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S14-144 T formed a lineage within the genus Nakamurella and shared the highest 16S rRNA gene sequence similarity with Nakamurella deserti 12Sc4-1 T (96.5 %) and Nakamurella silvestris S20-107 T (96.4 %). The average nucleotide identity value between the genomes of strain 14-144 T and the type strain of the species, N. deserti , was 72.0 % . The DNA G+C content of strain S14-144 T was 61.6 mol% . The major cellular fatty acids of strain S14-144 T were summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ) and C 16 : 0 . The strain contained MK-8(H4) as the predominant respiratory quinone, phosphatidylethanolamine and diphosphatidylglycerol as the major polar lipids, rhamnose, ribose and glucose as the major whole-cell sugars, and meso- diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. On the basis of the phylogenetic, phenotypic and chemotaxonomic analysis, strain S14-144 T is considered to represent a novel species of the genus Nakamurella , for which the name Nakamurella antarctica sp. nov. is proposed. The type strain is S14-144 T (=CCTCC AB 2015345T=KCTC 39796 T ).