Open AccessSphingorhabdus pulchriflava sp. nov., isolated from a river
Author(s) -
Gi-Yong Jung,
In-Hyun Nam,
Yong-Soo Han,
Joo Sung Ahn,
SungKeun Rhee,
So-Jeong Kim
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003503
Subject(s) - 16s ribosomal rna , biology , strain (injury) , oxidase test , phylogenetic tree , bacteria , glutaric acid , microbiology and biotechnology , fatty acid , gene , biochemistry , genetics , enzyme , anatomy
A facultatively anaerobic and Gram-stain-negative bacterium, strain GY_G T , was isolated from a river (Daedeock-cheon) in Daejeon, Republic of Korea. The isolate was catalase-positive, oxidase-positive and formed yellow colonies. Strain GY_G T was phylogenetically classified as belonging in the genus Sphingorhabdus . Its closely related strains were Sphingorhabdus wooponensis 03SU3-P T (97.1 % similarity), Sphingorhabdus buctiana T5 T (96.9 %), Sphingorhabdus contaminans JC216 T (96.5 %), Sphingorhabdus rigui 01SU5-P T (96.5 %) and Sphingorhabdus planktonica G1A_585 T (96.3 %) based on 16S rRNA gene sequences. The growth conditions for GY_G T were at 10-45 °C (optimum, 25 °C), pH 6-10 (pH 7) and 0-4% NaCl (0.5-1.5 %). Strain GY_G T could utilize turanose, d-fructose-6-phosphate, glucuronamide, α -keto-glutaric acid and acetoacetic acid. The major fatty acids of strain GY_G T were summed features 8 (C 18 : 1 ω 7 c /C 18 : 1 ω 6 c ; 40.6 %) and 3 (C 16 : 1 ω 6 c /C 16 : 1 ω 7 c ; 24.7 %). The major quinone required for respiration was Q-10. The polar lipids of strain GY_G T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and sphingolipid. The G+C content of the genome was 57.7 mol%. The average nucleotide identity and average amino acid identity values between strains GY_G T and S. wooponensis were 71.0 and 72.7 %, respectively. Based on phylogenetic and phenotypic attributes, we suggest that strain GY_G T is a novel species in the genus Sphingorhabdus and propose the name Sphingorhabdus pulchriflava . The type strain is GY_G T (=KCTC 62791 T =JCM 32855 T ).