Open AccessInhella crocodyli sp. nov., isolated from a crocodile pond
Author(s) -
Wen-Ming Chen,
Yi-Ru Xie,
Chiu-Chung Young,
Shih-Yi Sheu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003496
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , putrescine , gene , microbiology and biotechnology , botany , genetics , biochemistry , anatomy , enzyme
Strain CCP-18 T , isolated from a freshwater pond in Taiwan, was characterized by using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CCP-18 T belongs to the genus Inhella and has the highest levels of sequence similarity with respect to Inhella inkyongensis IMCC1713 T (98.9 %) and Inhella fonticola TNR-25 T (98.0 %). Cells were Gram-stain-negative, aerobic, motile, rod-shaped and formed white-coloured colonies. Optimal growth occurred at 25 °C, pH 6 and in the absence of NaCl. The major fatty acids of strain CCP-18 T were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c) and C16 : 0. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminophospholipids, an unidentified phospholipid, an unidentified aminolipid and an unidentified lipid. The predominant polyamine was putrescine. The major isoprenoid quinone was Q-8. The draft genome was approximately 3.76 Mb in size with a G+C content of 68.9 mol%. The DNA-DNA hybridization values for strain CCP-18 T with I.inkyongensis IMCC1713 T and I.nhella fonticola TNR-25 T were less than 40 %. Based on the phylogenetic and phenotypic data, strain CCP-18 T should be classified within the genus Inhella as a representative of a novel species, named Inhella crocodyli sp. nov. The type strain is CCP-18 T (=BCRC 81120 T =LMG 30595 T =KCTC 62511 T ).