Open AccessGeobacillus proteiniphilus sp. nov., a thermophilic bacterium isolated from a high-temperature heavy oil reservoir in China
Author(s) -
Е. М. Семенова,
Diyana S. Sokolova,
Denis S. Grouzdev,
А. Б. Полтараус,
N. G. Vinokurova,
T. P. Tourova,
Т. Н. Назина
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003486
Subject(s) - thermophile , biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , bacteria , microbiology and biotechnology , gene , genome , genetics , anatomy
A rod-shaped, spore-forming, thermophilic, chemoorganotrophic, aerobic or facultatively anaerobic bacterial strain, 1017 T , was isolated from production water sampled at the Dagang oilfield (PR China), and was characterized by using a polyphasic approach. The strain is capable of anaerobic glucose fermentation. Nitrate is reduced to nitrite. Optimal growth was observed at 60-65 °C, at pH between pH 7.0 and 7.5, and with 1-2 % (w/v) NaCl. The major cellular fatty acids were iso-C 17 : 0 , anteiso-C 17 : 0 , iso-C 15 : 0 , iso-C 16 : 0 and C 16 : 0 . The predominant polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. Phylogenetic analysis based on the 16S rRNA, gyrB and parE gene sequences indicated that the isolate belonged to the genus Geobacillus and was most closely related to Geobacillus thermoleovorans KCTC 3570 T (99.5, 96.1 and 97.9 % sequence similarity, respectively). Genome sequencing revealed a genome size of 3.57495 Mb and a DNA G+C content of 51.8 mol%. The average nucleotide identity and digital DNA-DNA hybridization values between the genomes of strain 1017 T and G. thermoleovorans KCTC 3570 T were 95.9 and 64.9 %, respectively. Results of phylogenomic metrics analysis of the genome and 1172 core genes of strain 1017 T and its physiological and biochemical characteristics confirmed that strain 1017 T represented a novel species of the genus Geobacillus , for which the name Geobacillusproteiniphilus sp. nov. is proposed. The type strain is 1017 T (=VKM B-3132 T =KCTC 33986 T ).