Open AccessDeinococcus psychrotolerans sp. nov., isolated from soil on the South Shetland Islands, Antarctica
Author(s) -
Jin Tian,
Liqiu Wang,
Ping Liu,
Yingchao Geng,
Guoxin Zhu,
Ruichen Zheng,
Zuobing Liu,
Yiming Zhao,
Jian Yang,
Fang Peng
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003484
Subject(s) - deinococcus , biology , diamino acid , 16s ribosomal rna , strain (injury) , phylogenetic tree , phylogenetics , ribosomal rna , coccus (insect) , genus , peptidoglycan , bacteria , microbiology and biotechnology , glycolipid , botany , genetics , gene , biochemistry , deinococcus radiodurans , gene sequence , anatomy
A Gram-stain-negative, non-motile, strictly aerobic, coccus-shaped bacterium, designated S14-83 T , was isolated from a soil sample collected from the South Shetland Islands of Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain is a novel member of the genus Deinococcus , with Deinococcus alpinitundrae as its closest relative (96.1 % similarity). The DNA G+C content of the strain was 61.1 mol% and the major respiratory quinone was MK-8. Major cellular fatty acids were summed feature 3 (C 16 : 1 ω7 c /C 16 : 1 ω6 c ) and C 16 : 0 . As well as containing glycophospholipid, aminophospholipids and glycolipid as major polar lipids, there were also some unknown polar lipids. The diagnostic diamino acid in the cell-wall peptidoglycan was ornithine, corroborating the assignment of the strain to the genus Deinococcus . Strain S14-83 T was shown to be extremely resistant to gamma radiation (>10 kGy) and UV light (460 Jm -2 ). On the basis of phylogenetic, chemotaxonomic and phenotypic data presented here, strain S14-83 T represents a novel species of the genus Deinococcus , for which the name Deinococcus psychrotolerans sp. nov. is proposed. The type strain is S14-83 T (=CCTCC AB 2015449 T = DSM 105285 T ).