Open AccessCorticimicrobacter populi gen. nov., sp. nov., a member of the family Alcaligenaceae, isolated from symptomatic bark of Populus × euramericana canker
Author(s) -
Yong Li,
Xue Han,
Dan-Ran Bian,
Lifang Gao,
Chungen Piao
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003483
Subject(s) - biology , 16s ribosomal rna , housekeeping gene , strain (injury) , bark (sound) , botany , phylogenetic tree , microbiology and biotechnology , gene , genetics , anatomy , ecology , gene expression
One Gram-negative aerobic bacterial strain was isolated from the bark tissue of Populus × euramericana and investigated using a polyphasic approach including 16S rRNA gene sequencing and both phenotypic and chemotaxonomic assays. The 16S rRNA gene and housekeeping gene phylogenies suggest that the novel isolate is different from the other genera of the family Alcaligenaceae. The G+C content, major fatty acids, physiological and biochemical data supported the distinctiveness of the novel strain from reference species. The major fatty acids detected in the novel isolate were C 16 : 1 ω7 c and/or C 16 : 1 ω6 c , C 16 : 0 , C 14 : 0 3OH and/or C 16 : 1 isoI and C 18 : 1 ω7 c . The phospholipid profile of strain d3-2-2 T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, aminolipid, aminophospholipid and an unidentified lipid. The quinone of the novel isolate was Q-8. Therefore, based on the data, the strain constitutes a novel species of a novel genus within the family Alcaligenaceae , for which the name Corticimicrobacter populi gen. nov., sp. nov. is proposed. The type strain is 3d-2-2 T (=CFCC 11891 T =KCTC 52807 T ).