Open AccessNeisseria weixii sp. nov., isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae)
Author(s) -
Zhilun Gui,
Jing Yang,
XinHe Lai,
Dong Jin,
Ji Pu,
Xiangning Bai,
Yanwen Xiong,
Zhihong Ren,
Xu Luo,
Ying Huang,
Shan Lu,
Jianguo Xu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003466
Subject(s) - biology , neisseria , 16s ribosomal rna , microbiology and biotechnology , phylogenetic tree , pika , bacteria , gene , genetics , ecology , national park
Three independent isolates (10022 T , 10 009 and 10011) of a novel catalase-positive, Gram-stain-negative coccus in the genus Neisseria were obtained from the rectal contents of plateau pika on the Qinghai-Tibet Plateau, PR China. Based on 16S rRNA gene sequence analysis, our newly identified organisms were most closely related to Neisseria iguanae, Neisseria flavescens and Neisseria perflava with similarities ranging from 98.02 to 98.45 %, followed by seven other species in the genus Neisseria. Phylogenetic analysis based on 16S rRNA and rplF genes showed that our three novel isolates group with members of the genus Neisseria. Results of the average nucleotide identity (ANI) analysis confirmed that our isolates are of the same species, and the ANI values between type strain 10022 T and other Neisseria species are 74.12-85.06 %, lower than the threshold range of 95-96 %. The major cellular fatty acids for our novel species are C16 : 0 and C16:1ω7c/C16:1ω6c, which along with their phenotypic characteristics can distinguish our isolates from other Neisseria species. On the basis of polyphasic analyses, our isolates are proposed to represent a novel species in genus Neisseria, with the name Neisseria weixii sp. nov. The type strain is 10022 T (=DSM 103441 T =CGMCC 1.15732 T ).