Open AccessLactococcus allomyrinae sp. nov., isolated from gut of larvae of Allomyrina dichotoma
Author(s) -
Jun Heo,
Hayoung Cho,
Tomohiko Tamura,
Satomi Saitou,
Kwan-Ho Park,
Jeong-Seon Kim,
SeungBeom Hong,
Soon-Wo Kwon,
Soojin Kim
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003461
Subject(s) - biology , 16s ribosomal rna , rpob , peptidoglycan , microbiology and biotechnology , lactococcus lactis , strain (injury) , sequence analysis , bacteria , gene , genetics , lactic acid , anatomy
Strain 1JSPR-7 T , a facultatively anaerobic bacterium isolated from the gut of larvae of Allomyrina dichotoma raised in Wanju-gun, Republic of Korea, was characterized using a polyphasic approach. Comparative analysis of 16S rRNA gene and rpoB gene sequences showed that strain 1JSPR-7 T fell within the genus Lactococcus , forming a compact cluster with the type strain of four subspecies of Lactococcus lactis and Lactococcus taiwanensis . The 16S rRNA gene sequence of strain 1JSPR-7 T revealed the highest homology with L. lactis subsp. lactis JCM 5805 T (97.3 %) and L. lactis subsp. hordniae NBRC 100931 T (97.1 %), and the rpoB gene sequence showed the highest similarity to L. lactis subsp. cremoris DSM 20069 T (91.4 %) and L. lactis subsp. tructae L105 T (91.4 %). The average nucleotide identity and digital DNA-DNA hybridization values indicated that strain 1JSPR-7 T was a novel species of the genus Lacococcus . The major fatty acids (>10 % of the total fatty acids) were summed feature 7 (unknown 18.846, C 19 : 1 ω6 c and/or C 19 : 0 cyclo ω10 c ), C 16 : 0 and C 14 : 0 , and the predominant menaquinone was MK-8 with MK-7 as a minor one. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and three unidentified glycolipids with diphosphatidylglycerol as the major one. The cell-wall peptidoglycan was of the A4 α type with an interpeptide bridge comprising l-Lys-d-Asp. The DNA G+C content based on the whole genome sequences was 37.4 mol%. Based on the data obtained, strain 1JSPR-7 T represents a novel species of the genus Lactococcus , for which the name Lactococcusallomyrinae sp. nov. is proposed. The type strain is 1JSPR-7 T (=KACC 19319 T =NBRC 113068 T ).