Open AccessVagococcus bubulae sp. nov., isolated from ground beef, and Vagococcus vulneris sp. nov., isolated from a human foot wound
Author(s) -
Patricia L. Shewmaker,
Anne M. Whitney,
Christopher A. Gulvik,
Ben W. Humrighouse,
Jarrett Gartin,
Hércules Moura,
John R. Barr,
Edward R. B. Moore,
Roger Karlsson,
Tatiana Castro Abreu Pinto,
Lúcia Martins Teixeira
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003459
Subject(s) - biology , microbiology and biotechnology , rpob , 16s ribosomal rna , gene , genetics
Two unusual catalase-negative, Gram-stain-positive, Vagococcus-like isolates that were referred to the CDC Streptococcus Laboratory for identification are described. Strain SS1994 T was isolated from ground beef and strain SS1995 T was isolated from a human foot wound. Comparative 16S rRNA gene sequence analysis of isolates SS1994 T and SS1995 T against Vagococcus type strain sequences supported their inclusion in the genus Vagococcus. Strain SS1994 T showed high sequence similarity (>97.0 %) to the two most recently proposed species, Vagococcus martis (99.2 %) and Vagococcus teuberi (99.0 %) followed by Vagococcus penaei (98.8 %), strain SS1995 T (98.6 %), Vagococcus carniphilus (98.0 %), Vagococcus acidifermentans (98.0 %) and Vagococcus fluvialis (97.9 %). The 16S rRNA gene sequence of strain SS1995 T was most similar to V. penaei (99.1 %), followed by SS1994 T (98.6 %), V. martis (98.4 %), V. teuberi (98.1 %), V. acidifermentans (97.8 %), and both V. carniphilus and V. fluvialis (97.5 %). A polyphasic taxonomic study using conventional biochemical and the rapid ID 32 STREP system, MALDI-TOF MS, cell fatty acid analysis, pairwise sequence comparisons of the 16S rRNA, rpoA, rpoB, pheS and groL genes, and comparative core and whole genome sequence analyses revealed that strains SS1994 T and SS1995 T were two novel Vagococcus species. The novel taxonomic status of the two isolates was confirmed with core genome phylogeny, average nucleotide identity <84 % and in silico DNA-DNA hybridization <28 % to any other Vagococcus species. The names Vagococcusbubulae SS1994 T =(CCUG 70831 T =LMG 30164 T ) and Vagococcusvulneris SS1995 T =(CCUG 70832 T =LMG 30165 T ) are proposed.