Open AccessLeucobacter muris sp. nov., isolated from the nose of a laboratory mouse
Author(s) -
Laurentiu Benga,
Cathrin Spröer,
Peter Schümann,
Susanne Verbarg,
Boyke Bunk,
Eva Engelhardt,
W. Peter M. Benten,
Martin Sager
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003446
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , strain (injury) , microbiology and biotechnology , genomic dna , phylogenetic tree , gene , sequence analysis , genetics , anatomy
A Gram-stain-positive, rod-shaped, aerobic, non-motile, white, opaque bacterial isolate, designated 924/12 T , was isolated from the nose of a laboratory mouse in Düsseldorf, Germany. The 16S rRNA gene sequence analyses indicated the phylogenetic position of the strain within the genus Leucobacter. Similarity levels over 97 % were recorded between the 16S rRNA gene sequence of strain 924/12 T and the type strains of the species Leucobacter chironomi DSM 19883 T (99.5 %), followed by Leucobacter celersubsp. astrifaciens CBX151 T (97.6 %), Leucobacter celersubsp. celer NAL101 T (97.5 %), 'Leucobacter kyeonggiensis' F3-P9 (97.5 %), Leucobacter zeae CC-MF41 T (97.3 %), Leucobacter chromiiresistens JG31 T (97.1 %), Leucobacter triazinivorans JW-1 T (97.1 %), Leucobacter corticis 2 C-7 T (97.0 %) and Leucobacter aridicolis CIP108388 T (97.0 %). DNA-DNA hybridization and whole genomic comparison, mandatory to taxonomically separate strain 924/12 T from the type strain of L. chironomi, revealed similarity values of 40.4 and 30.8 %, respectively, thus below the threshold of 70 % recommended differentiating between species. The cell-wall amino acids of the novel isolate were diaminobutyric acid, alanine, glycine, threonine and glutamic acid. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unknown lipid, whereas the predominant menaquinones were MK-11 and MK-10. The genomic DNA G+C content of strain 924/12 T was 70.6 mol%. Phylogenetic analyses based on the 16S rRNA gene sequences and the phenotypical differences between strain 924/12 T and the other closely related type strains of the genus Leucobacter indicated that strain 924/12 T represents a novel species within the genus Leucobacter, family Microbacteriaceae, for which the name Leucobacter muris sp. nov. is proposed. The type strain is 924/12 T (=DSM 101948 T =CCM 8761 T ).