Open AccessRunella aurantiaca sp. nov., isolated from sludge of a manganese mine
Author(s) -
XiongLi Yang,
Zijie Zhou,
Shuijiao Liao,
Gejiao Wang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003435
Subject(s) - 16s ribosomal rna , biology , strain (injury) , glycolipid , gene , microbiology and biotechnology , bacteria , halophile , bacteroidetes , thermophile , botany , biochemistry , genetics , anatomy
A Gram-stain-negative, filamentous rod-shaped, aerobic and non-motile strain, YX9 T , was isolated from sludge of a manganese mine. Analysis of the 16S rRNA gene sequence revealed that strain YX9 T formed the same branch within the members of the genus Runella and showed high relatedness to Runella slithyformis DSM 19594 T (98.1 %), Runella palustris HMF3829 T (96.0 %) and Runella zeae NS12 T (95.4 %). The genome length of strain YX9 T was 7.21 Mb, had 5985 coding sequences and a DNA G+C content of 44.8 mol%. The average nucleotide identity value of the draft genomes between strain YX9 T and R . slithyformis DSM 19594 T was 80.7 %. The major fatty acids of strain YX9 T were iso-C 15 : 0 , C 16:1 ω 5 c and summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ). The predominant respiratory quinone was menaquinone 7. The polar lipids of strain YX9 T were phosphatidylethanolamine, four unidentified lipids, two aminolipids, a phospholipid and a glycolipid. Based on the results of genotypic and phenotypic studies, strain YX9 T represents a novel species within the genus Runella , for which the name Runella aurantiaca sp. nov. is proposed (=KCTC 62875 T =CCTCC AB 2018214 T ).