Open AccessRhizobium chutanense sp. nov., isolated from root nodules of Phaseolus vulgaris in China
Author(s) -
Yunyun Huo,
Wenjun Tong,
Juanjuan Wang,
Fang Wang,
Wenqing Bai,
En Tao Wang,
Peng Shi,
Weimin Chen,
Gehong Wei
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003430
Subject(s) - phaseolus , biology , rhizobium , botany , china , root nodule , symbiosis , horticulture , bacteria , inoculation , political science , law , genetics
Two Gram-stain-negative, rod-shaped bacterial strains (C5 T and C16), isolated from root nodules of Phaseolus vulgaris L. in Jiangxi Province, PR China, were characterized by using a polyphasic taxonomical approach. The phylogenetic analysis of the 16S rRNA gene and three concatenated housekeeping genes (recA-glnII-atpD) revealed that C5 T and C16 were members of the genus Rhizobium, yet were distinct from known species. The case for strain C5 T representing a novel species was supported by genomic results. Pairwise digital DNA-DNA hybridization and average nucleotide identity values were much lower than the proposed and generally accepted species boundaries. The genome-based phylogenetic tree reconstructed by using the up-to-date bacterial core gene set consisting of 92 genes showed that the strains formed a monophyletic branch, further supporting this result. The symbiotic genes of nodC and nifH were identified in both strains; each could nodulate Phaseolus vulgaris and Glycine max but not Leucaena leucocephala, Pisum sativum or Medicago sativa plants. Major cellular fatty acids of C5 T were summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c; 58.8 %), C18 : 1 ω7c 11-methyl (14.2 %) and C18 : 0 (8.1 %). The DNA G+C content of C5 T was 61.4 mol%. Based on these genomic, chemotaxonomic and phenotypic characteristics, we propose a novel species: Rhizobium chutanense sp. nov. The type strain is C5 T (=CCTCC AB 2018143 T =LMG 30777 T ).