Open AccessYersinia hibernica sp. nov., isolated from pig-production environments
Author(s) -
Scott V. Nguyen,
Dechamma Mundanda Muthappa,
Daniel Hurley,
Orla Donoghue,
Evonne McCabe,
João Anes,
Kirsten Schaffer,
Brenda Murphy,
Jim Buckley,
Séamus Fanning
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003422
Subject(s) - biology , yersinia enterocolitica , 16s ribosomal rna , phylogenetic tree , yersinia , strain (injury) , microbiology and biotechnology , genome , genetics , gene , bacteria , anatomy
A Gram-stain-negative, rod-shaped strain isolated from pig-production environments was identified as a new species within the genus Yersinia using multifaceted genomic and biochemical approaches. The genome of this strain was closed using a hybrid assembly approach combining both high accuracy short read sequencing data with long read sequencing technology. Phylogenetic analysis of the 16S rRNA gene showed ~98 % similarity to Yersinia kristensenii and ~98 % similarity to Yersinia enterocolitica. Average nucleotide identity (OrthoANI) values were calculated as 85.79 % to Y. kristensenii ATCC 33638 T and 85.73 % to Y. enterocolitica ATCC 9610 T thereby providing evidence that this isolate should be considered as a novel species. The type strain is CFS1934 T (=NCTC 14222 T =LMG 31076 T ).