Open AccessAzotobacter bryophylli sp. nov., isolated from the succulent plant Bryophyllum pinnatum
Author(s) -
Lihui Liu,
Tao Yuan,
Qianli An,
Meiyan Yang,
Xiaoyun Mao,
Mo Chen,
Zhiyuan Tan,
Guixiang Peng
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003412
Subject(s) - biology , botany , azotobacter , bacteria , genetics
A Gram-stain-negative, aerobic, nitrogen-fixing bacterium, designated strain L461 T , was isolated from leaves of Bryophyllum pinnatum growing at the South China Agricultural University. Phylogenetic analysis of the 16S rRNA gene sequence indicated it as a member of the genus Azotobacter closely related to Azotobacter beijerinckii JCM 20725 T (97.82 % similarity) and Azotobacter chroococcum ATCC 9043 T (97.34 %). Its major fatty acid components were C16 : 1 ω9c and C16 : 0. Its predominant isoprenoid quinone was Q-9. Its major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid, phospholipid and one unknown lipid. Its DNA G+C content was 64.9 mol% (Tm). DNA-DNA relatedness values between strain L461 T and the reference strains of A. beijerinckii and A. chroococcum were 46.43 and 28.23 %, respectively. Biological and biochemical tests, protein patterns, genomic DNA fingerprinting, and comparison of cellular fatty acids distinguished strain L461 T from the closely related Azotobacter species. Based on these data, the novel species Azotobacter bryophylli sp. nov. is proposed, with the type strain L461 T (=KCTC 62195 T =GDMCC 1.1250 T ).