Open AccessSneathiella limimaris sp. nov., a marine alphaproteobacterium isolated from a tidal mudflat and emended description of the genus Sneathiella
Author(s) -
Soon Dong Lee
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003411
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , microbiology and biotechnology , ribosomal rna , genus , bacteria , phylogenetics , gene , oxidase test , botany , zoology , genetics , biochemistry , anatomy , enzyme
A novel marine bacterium, designated strain GH1-24 T , was isolated from a tidal mudflat sample collected at Gangwha Island, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequence comparison exhibited that the novel isolate was most closely related to Sneathiella and formed a tight cluster with Sneathiella glossodoripedis JCM 23214 T with 98.1 % sequence similarity. The 16S rRNA gene sequence similarities to other Sneathiella species were low: Sneathiella chinensis LMG 23452 T (96.1 %) and Sneathiellachungangensis CAU 1294 T (95.8 %). Cells of the organism were Gram-reaction-negative, strictly aerobic, catalase- and oxidase-positive, motile rods and showed growth at 0.5-6 % NaCl, 20-42 °C and pH 6.0-8.0. The G+C content of the DNA was 54.9 mol%. The major isoprenoid quinone was Q-10. The major cellular fatty acids were C18 : 1ω7c, C16 : 0 and C19 : 0cyclo ω8c. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospholipid. The results of phenotypic and phylogenetic analyses and DNA-DNA relatedness studies suggest that the isolate can be a member of a novel species of the genus Sneathiella, for which the name Sneathiellalimimaris sp. nov. is proposed. The type strain is GH1-24 T (=KCTC 52846 T =NBRC 113276 T ).