Open AccessAnaerosacchariphilus polymeriproducens gen. nov., sp. nov., an anaerobic bacterium isolated from a salt field
Author(s) -
Wonduck Kim,
SungHyun Yang,
Mi-Jeong Park,
Jihye Oh,
Jung-Hyun Lee,
Kae Kyoung Kwon
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003404
Subject(s) - biology , 16s ribosomal rna , strain (injury) , cellobiose , bacteria , lachnospiraceae , botany , microbiology and biotechnology , biochemistry , firmicutes , hydrolysis , genetics , cellulase , anatomy
A Gram-stain-negative oval-rod-shaped, spore-forming anaerobic bacterium, designated as strain MCWD5 T , was isolated from sediment of a salt pond in the Republic of Korea (35° 7' 18″ N 126° 19' 4″ E). The 16S rRNA gene sequence analysis revealed that strain MCWD5 T had low similarity values to members in the family Lachnospiraceae, such as Robinsoniella peoriensis PPC31 T (94.8 %), Ruminococcusgauvreauii CCRI-16110 T (94.2 %) and Lachnotalea glycerini DLD10 T (94.0 %), and its phylogenetic position is unstable. The strain could grow at 20-42 °C (optimum, 38-42 °C), pH 5.5-10.0 (pH 7.0) and with 0-6 % (2.0 %) NaCl. Strain MCWD5 T could not use nitrate, nitrite, sulfate or sulfite as electron acceptors. The strain could utilize various carbohydrates, such as arabinose, cellobiose, glucose, etc., and polymers such as pectin and starch. The major fatty acids of strain MCWD5 T were C14 : 0, C16 : 0, C16 : 1ω7c, C18 : 1ω7c DMA and summed feature 8 (C17 : 1ω8c and/or C17 : 2), which was clearly different from those of related genera. The major polar lipids were diphosphatidyglycerol, phosphatidyglycerol and an unknown phospholipid. Based on the results of phylogenetic, physiologic and chemotaxonomic studies, Anaerosacchariphilus polymeriproducens gen. nov., sp. nov. with the type strain MCWD5 T (=KCTC 15595 T =DSM 105757 T ) is proposed in the family Lachnospiraceae.