Open AccessPedobacter chinensis sp. nov., a cellulose-decomposing bacterium from Arctic tundra soil
Author(s) -
Lichun Zhou,
Xuyang Chen,
ZongJun Du,
DaShuai Mu
Publication year - 2019
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003403
Subject(s) - 16s ribosomal rna , biology , strain (injury) , bacteria , tundra , tidal flat , microbiology and biotechnology , botany , arctic , genetics , ecology , sediment , paleontology , anatomy
A novel bacterial strain, JDX94 T , was isolated from tundra soil sampled north of the Yellow River station, Arctic. Cells were Gram-stain-negative, non-spore-forming, short rod-shaped and aerobic. The strain displayed growth at 4-37 °C with an optimum at 28 °C, with 0-1.0 % (w/v) NaCl (optimum, 0%) and at pH 6.0-9.0 (optimum, pH 7.0-7.5). Cells contained summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and iso-C17 : 0 3-OH as its major cellular fatty acids and menaquinone-7 as the only respiratory quinone. The polar lipid profile of strain JDX94 T consisted of phosphatidylethanolamine, two unidentified aminolipids and four unknown polar lipids. The DNA G+C content was 37.5 mol%. On the basis 16S rRNA gene sequence comparison, strain JDX94 T showed the highest sequence similarity (96.7 %) to Pedobacteragri JCM 15120 T , followed by Pedobacteralluvionis DSM 19624 T (96.3 %). Furthermore, the average nucleotide identity and digital DNA-DNA hybridization values between strain JDX94 T and related species of the genus Pedobacter were 74.6-79.2 % and 18.9-24.5 %, respectively. Based on the presented results, we propose a novel species for which the name Pedobacterchinensis sp. nov. is suggested, with the type strain JDX94 T (=MCCC 1H00335 T = KCTC 62850 T ).